Application of Novel Sialoglyco Particulates Enhances the Detection Sensitivity of the Equine Influenza Virus by Real-Time Reverse Transcriptase Polymerase Chain Reaction

Makoto Ogata; Takashi Yamanaka; Ami Koizumi; Mao Sakamoto; Rena Aita; Hiroyuki Endo; Takehiro Yachi; Noriko Yamauchi; Tadamune Otsubo; Kiyoshi Ikeda; Tatsuya Kato; Enoch Y Park; Hiroyuki Kono; Manabu Nemoto; Kazuya I P J Hidari

doi:10.1021/acsabm.8b00813

Application of Novel Sialoglyco Particulates Enhances the Detection Sensitivity of the Equine Influenza Virus by Real-Time Reverse Transcriptase Polymerase Chain Reaction

ACS Appl Bio Mater. 2019 Mar 18;2(3):1255-1261. doi: 10.1021/acsabm.8b00813. Epub 2019 Feb 11.

Authors

Affiliations

¹ Department of Applied Chemistry and Biochemistry, National Institute of Technology, Fukushima College, 30 Nagao, Iwaki, Fukushima 970-8034, Japan.
² Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
³ Department of Materials Science and Engineering, Graduate School of Science and Engineering, Ibaraki University, 4-12-1 Naka-narusawa-cho, Hitachi, Ibaraki 316-8511, Japan.
⁴ Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University, Kure-shi, Hiroshima, Japan.
⁵ Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
⁶ Division of Applied Chemistry and Biochemistry, National Institute of Technology, Tomakomai College, Nishikioka 443, Tomakomai, Hokkaido 059-1275, Japan.
⁷ Department of Food and Nutrition, Junior College Division, University of Aizu, 1-1 Aza-Kadota, Yahata, Ikki-machi, Aizuwakamatsu, Fukushima 965-8570, Japan.

PMID: 35021374
DOI: 10.1021/acsabm.8b00813

Abstract

Sialoglyco particulates carrying an N-glycolylneuraminyl-α-(2 → 3)-N-acetyllactosamine (Neu5Gcα2,3LacNAc) residue that displays a high level of affinity for the equine influenza virus (EIV) were generated using sialoglycopolypeptide and hexyl-containing hybrid silica particulates. The particulates were spherical with a diameter of approximately 950 nm and found to have good dispersibility in aqueous solution. Interaction between the sialoglyco particulates and the EIV was investigated by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) of the EIV genome captured on the particulates. The number of EIV-specific genes detected by rRT-PCR on a nasal swab obtained from infected horses clearly increased when the sample was treated with sialoglyco particulates. Our results show these novel sialoglyco particulates can be used as a highly sensitive tool for detecting low levels of EIV that were previously undetectable in the early or late stage of infection.

Keywords: carbohydrate; equine influenza virus; multivalency; particulate; sensitive detection.