Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

Anal Methods. 2022 Jan 27;14(4):378-382. doi: 10.1039/d1ay01395e.

Abstract

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

MeSH terms

  • COVID-19*
  • Colorimetry
  • Humans
  • Molecular Diagnostic Techniques
  • Nucleic Acid Amplification Techniques
  • Pandemics
  • RNA, Viral / genetics
  • Reproducibility of Results
  • SARS-CoV-2*

Substances

  • RNA, Viral

Supplementary concepts

  • LAMP assay