Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

STAR Protoc. 2021 Dec 23;3(1):101060. doi: 10.1016/j.xpro.2021.101060. eCollection 2022 Mar 18.

Abstract

Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).

Keywords: Immunology; Microscopy; Model Organisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Intravitreal Injections
  • Lens, Crystalline*
  • Macrophage Activation
  • Macrophage Colony-Stimulating Factor* / pharmacology
  • Mice
  • Vitreous Body

Substances

  • Macrophage Colony-Stimulating Factor