The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)

Rachel E T Bentley; Charles C T Hindmarch; Kimberly J Dunham-Snary; Brooke Snetsinger; Jeffrey D Mewburn; Arthur Thébaud; Patricia D A Lima; Bernard Thébaud; Stephen L Archer

doi:10.1016/j.dib.2021.107736

The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)

Data Brief. 2021 Dec 20:40:107736. doi: 10.1016/j.dib.2021.107736. eCollection 2022 Feb.

Authors

Rachel E T Bentley¹, Charles C T Hindmarch^{1

2}, Kimberly J Dunham-Snary^{1

3}, Brooke Snetsinger², Jeffrey D Mewburn³, Arthur Thébaud⁴, Patricia D A Lima², Bernard Thébaud^{5

6

7

8}, Stephen L Archer^{1

2}

Affiliations

¹ Department of Medicine, Queen's University, Kingston, Ontario, Canada.
² QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada.
³ Department of Biomedical and Molecular Science, Queen's University, Canada.
⁴ Department of Kinesiology and Health Studies, Queen's University, Canada.
⁵ Ottawa Hospital Research Institute, Regenerative Medicine Program, Ottawa, Ontario, Canada.
⁶ Department of Pediatrics, Division of Neonatology, Children's Hospital of Eastern Ontario, Ottawa, Ontario, Canada.
⁷ Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa, Ontario, Canada.
⁸ Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

Abstract

The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O₂), in the first few days of life, the DA responds with a functional closure that is followed by anatomical closure. Here, we study human DA smooth muscle cells (DASMC) taken from 10 term infants during congenital heart surgery. Purification of these cells using flow cytometry ensured a pure population of DASMCs, which we confirmed as responsive to O₂. An oxygen-induced increase in intracellular calcium of 18.1%±4.4% and SMC constriction (-27%±1.5% shortening) occurred in all cell lines within five minutes. These cells were maintained in either hypoxia (2.5% O₂), mimicking in utero conditions or in normoxia (19% O₂) mimicking neonate conditions. We then used 3' RNAsequencing to identify the transcriptome of DASMCs in each condition [1]. In this paper, we present the full differentially regulated gene list from this experiment.

Keywords: Hypoxia; Normoxia; Oxygen sensing; Patent ductus arteriosus; RNAsequencing; Smooth muscle cells.

Abstract

Grants and funding