Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak; Ivan Kodvanj; Ana Babic Perhoc; Davor Virag; Ana Knezovic; Jelena Osmanovic Barilar; Peter Riederer; Melita Salkovic-Petrisic

doi:10.1016/j.mex.2021.101611

Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

MethodsX. 2021 Dec 22:9:101611. doi: 10.1016/j.mex.2021.101611. eCollection 2022.

Authors

Jan Homolak^{1

2}, Ivan Kodvanj^{1

2}, Ana Babic Perhoc^{1

2}, Davor Virag^{1

2}, Ana Knezovic^{1

2}, Jelena Osmanovic Barilar^{1

2}, Peter Riederer^{3

4}, Melita Salkovic-Petrisic^{1

2}

Affiliations

¹ Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia.
² Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia.
³ Center of Mental Health, Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital Würzburg, Würzburg, Germany.
⁴ Department and Research Unit of Psychiatry, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark.

Abstract

We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.•NRP is a rapid, cost-effective, and simple method for reductive capacity assessment•NRP is compatible with a high-throughput screening of solid and liquid biological samples•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis.

Keywords: Antioxidant; Nitrocellulose; Oxidative stress; Potassium permanganate; ROS; Reductive capacity.