Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

Zhen Wang; Manoj Kumar Solanki; Zhuo-Xin Yu; Muhammad Anas; Deng-Feng Dong; Yong-Xiu Xing; Mukesh Kumar Malviya; Fei Pang; Yang-Rui Li

doi:10.3389/fmicb.2021.797889

Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

Front Microbiol. 2021 Dec 23:12:797889. doi: 10.3389/fmicb.2021.797889. eCollection 2021.

Authors

Zhen Wang^{1

2

3}, Manoj Kumar Solanki⁴, Zhuo-Xin Yu³, Muhammad Anas³, Deng-Feng Dong³, Yong-Xiu Xing³, Mukesh Kumar Malviya², Fei Pang¹, Yang-Rui Li^{2

3}

Affiliations

¹ Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, College of Biology and Pharmacy, Yulin Normal University, Yulin, China.
² Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Guangxi Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, China.
³ Agricultural College, Guangxi University, Nanning, China.
⁴ Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland.

Abstract

To understand the beneficial interaction of sugarcane rhizosphere actinobacteria in promoting plant growth and managing plant diseases, this study investigated the potential role of sugarcane rhizospheric actinobacteria in promoting plant growth and antagonizing plant pathogens. We isolated 58 actinobacteria from the sugarcane rhizosphere, conducted plant growth-promoting (PGP) characteristics research, and tested the pathogenic fungi in vitro. Results showed that BTU6 (Streptomyces griseorubiginosus), the most representative strain, regulates plant defense enzyme activity and significantly enhances sugarcane smut resistance by regulating stress resistance-related enzyme (substances (POD, PAL, PPO, TP) in sugarcane) activity in sugarcane. The genomic evaluation indicated that BTU6 has the ability to biosynthesize chitinase, β-1,3-glucanase, and various secondary metabolites and plays an essential role in the growth of sugarcane plants under biotic stress. Potential mechanisms of the strain in improving the disease resistance of sugarcane plants and its potential in biodegrading exogenous chemicals were also revealed. This study showed the importance of sugarcane rhizosphere actinobacteria in microbial ecology and plant growth promotion.

Keywords: actinobacteria; biocontrol; genome; smut; sugarcane.