A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry

Aleksandr S Taraskin; Konstantin K Semenov; Alexey A Lozhkov; Irina L Baranovskaya; Aleksandr V Protasov; Edward S Ramsay; Alexandr A Tyulin; Olga A Mirgorodskaya; Andrey V Vasin; Sergey A Klotchenko; Yana A Zabrodskaya

doi:10.1016/j.jpba.2021.114575

A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry

J Pharm Biomed Anal. 2022 Feb 20:210:114575. doi: 10.1016/j.jpba.2021.114575. Epub 2022 Jan 3.

Affiliations

¹ Smorodintsev Research Institute of Influenza, div. Russian Ministry of Health, 15/17 Ulitsa Professora Popova, St. Petersburg 197376, Russia; Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia.
² Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia.
³ Smorodintsev Research Institute of Influenza, div. Russian Ministry of Health, 15/17 Ulitsa Professora Popova, St. Petersburg 197376, Russia.
⁴ Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia; ITMO University, 49A Kronverksky Prospect, St.Petersburg 197101, Russia.
⁵ Smorodintsev Research Institute of Influenza, div. Russian Ministry of Health, 15/17 Ulitsa Professora Popova, St. Petersburg 197376, Russia; Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia; Petersburg Nuclear Physics Institute, B. P. Konstantinov of National Research Center, Kurchatov Institute, mkr. Orlova roshcha 1, Gatchina 188300, Russia. Electronic address: yana@zabrodskaya.net.

PMID: 34999434
DOI: 10.1016/j.jpba.2021.114575

Abstract

In this work, we have extended our previously proposed approach for determining protein concentrations in human serum (using MALDI-TOF mass spectrometry) to include simultaneous analysis of several proteins associated with acute inflammation (alpha-2-macroglobulin, fetuin-A, serum amyloid A1). This technique can be used to diagnose systemic inflammation and provides results in 4-5 h. The developed approach was verified using standard immunological methods (ELISA). Samples from 87 individuals, in specific groups, were used for testing and validation: control; inflammatory soft tissue disease accompanied by sepsis; influenza A infection; or COVID-19. The feasibility of differentiating patient groups with the aforementioned conditions was analyzed using a combination of the inflammatory markers described. For fetuin-A and serum amyloid A1, diagnostically significant concentration ranges were established.

Keywords: (18)O isotope exchange; Alpha-2-macroglobulin; Fetuin-A; MALDI MS; Quantitative mass spectrometry; Serum amyloid A.

MeSH terms

Biomarkers
COVID-19*
Humans
SARS-CoV-2
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Biomarkers