How do environmentally friendly antifouling alkaloids affect marine fouling microbial communities?

Yumiao Gao; Qingyue Meng; Xuefeng Zhou; Xiaowei Luo; Ziheng Su; Zihui Chen; Riming Huang; Yonghong Liu; Xiaoyong Zhang

doi:10.1016/j.scitotenv.2021.152910

How do environmentally friendly antifouling alkaloids affect marine fouling microbial communities?

Sci Total Environ. 2022 May 10:820:152910. doi: 10.1016/j.scitotenv.2021.152910. Epub 2022 Jan 6.

Authors

Yumiao Gao¹, Qingyue Meng², Xuefeng Zhou³, Xiaowei Luo⁴, Ziheng Su², Zihui Chen², Riming Huang⁵, Yonghong Liu⁶, Xiaoyong Zhang⁷

Affiliations

¹ University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; School of Biological Sciences, University of Edinburgh, Edinburgh EH93FL, United Kingdom.
² University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
³ Key Laboratory of Tropical Marine Bio-resources and Ecology, South China sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
⁴ Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China.
⁵ College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
⁶ Key Laboratory of Tropical Marine Bio-resources and Ecology, South China sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China. Electronic address: yonghongliu@scsio.ac.cn.
⁷ University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: zhangxiaoyong@scau.edu.cn.

PMID: 34999079
DOI: 10.1016/j.scitotenv.2021.152910

Abstract

Most previous studies on antifouling (AF) agents have focused on the influence of biofilm formation constituted by single or multiple cultured microbial species, and very few studies have analyzed the relationship between environmentally friendly AF compounds and marine fouling microbial communities (MFMCs). This is the first investigation of the impact of three environmentally friendly alkaloids (5-chlorosclerotiamide, circumdatin F and notoamide C) produced by the deep-sea-derived fungus Aspergillus westerdijkiae on MFMCs using high-throughput Illumina sequencing in a field test. The results of this study showed that the three alkaloids could significantly decrease the coverage of marine microflora (p < 0.05) and affect the composition and diversity of MFMCs on polyvinyl chloride (PVC) plates. Furthermore, 5-chlorosclerotiamide and notoamide C could completely inhibit many macrofouler-inductive-bacteria, such as Pseudoalteromonas and Pseudomonas, and promote the anti-macrofouler-bacteria, such as Winogradskyella, from 0.21% to more than 10% of the MFMCs on PVC plates. These results suggested that 5-chlorosclerotiamide and notoamide C could influence the compositions of MFMCs and make it unfavorable for the settlement of macrofoulers, by reducing the abundance of macrofouler-inductive-bacteria and promoting the percentage of anti-macrofouler-bacteria on PVC plates. The present study provides a new way to evaluate the effect of environmentally friendly AF compounds and obtain a better understanding of the antifouling process.

Keywords: Antifouling alkaloids; Deep-sea-derived fungi; Environmentally friendly; Marine fouling microbial communities.

MeSH terms

Alkaloids* / pharmacology
Bacteria
Biofilms
Biofouling* / prevention & control
Fungi
Microbiota*

Substances

Alkaloids