Microbial peroxide producing cell mediated lignin valorization

Abstract

Lignin is one of the most abundant naturally occurring polymers and can produce value-added products such as vanillin and p-coumaric acid. In the current work, in-situ depolymerization of lignin for its valorization in a microbial peroxide-producing cell (MPPC) system was performed. It is an electrochemical cell that requires no external energy to produce H₂O₂ for the advanced oxidation process. Lignin in the MPPC system undergoes oxidative depolymerization to generate value-added products. The maximum open-circuit voltage (OCV) was 1.143 V, the current density and power densities were 14 mA/cm² and 13 mW/cm², respectively, along with the production of 26 mM of H₂O₂. The degradation of signature linkages such as β-β bond and β-0-4 bond were analyzed and confirmed using FTIR spectroscopy. Vanillin, p-coumaric acid, ferulic acid, etc. were generated during depolymerization and were detected using LC-QTOF-MS.

Keywords: Fenton reaction; Lignin valorization; Microbial peroxide producing cells.