Escherichia coli ST471 Producing VIM-4 Metallo-β-Lactamase in Colombia

Ana Mercedes Rada; Adriana Correa; Eliana Restrepo; Cesar Capataz

doi:10.1089/mdr.2021.0031

Escherichia coli ST471 Producing VIM-4 Metallo-β-Lactamase in Colombia

Microb Drug Resist. 2022 Mar;28(3):288-292. doi: 10.1089/mdr.2021.0031. Epub 2022 Jan 4.

Authors

Ana Mercedes Rada^{1

2}, Adriana Correa^{3

4}, Eliana Restrepo², Cesar Capataz⁵

Affiliations

¹ Department of Microbiology, Bacteria and Cáncer Group, University of Antioquia, Medellín, Colombia.
² Facultad de Ciencias de la Salud, Biociencias Group, Institución Universitaria Colegio Mayor de Antioquia, Medellín, Colombia.
³ Facultad de Ciencias Básicas, Universidad Santiago de Cali, Cali, Colombia.
⁴ Clínica Imbanaco, Cali, Colombia.
⁵ Fundación Clínica del Norte, Bello, Colombia.

Abstract

An Escherichia coli isolate sequence-type 471 (ST471) producing Verona integron-encoded metallo-β-lactamases (VIM)-4 was recovered from a rectal swab in a patient without travel records with osteomyelitis in Colombia. The isolate carried a class 1 integron-borne bla_VIM-4 gene with a 170-bp duplication in the 3' end of the gene, preceded by an aac(6')-Ib gene. The genetic environment of bla_VIM-4, bla_CMY-2, and sul2 genes showed similarities to the backbone of pKKp4, an IncA/C-type plasmid from a Klebsiella pneumoniae strain carrying bla_VIM-4 recovered in Kuwait. This is the first report of bla_VIM-4 in Enterobacterales in South America. Our results suggest that bla_VIM-4 gene was found on an IncA/C-type plasmid that could play a role in the spread of VIM-4 carbapenemase in Colombia.

Keywords: Escherichia coli; blaVIM-4; colonization; metallo-β-lactamase.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Colombia
Drug Resistance, Multiple, Bacterial
Escherichia coli / drug effects*
Escherichia coli / genetics*
Genes, Bacterial
Humans
Male
Microbial Sensitivity Tests
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
beta-Lactamases