Genome-wide DNA N6-adenine methylation in sea buckthorn (Hippophae rhamnoides L.) fruit development

Guoyun Zhang; Songfeng Diao; Yating Song; Caiyun He; Jianguo Zhang

doi:10.1093/treephys/tpab177

Genome-wide DNA N6-adenine methylation in sea buckthorn (Hippophae rhamnoides L.) fruit development

Tree Physiol. 2022 Jun 9;42(6):1286-1295. doi: 10.1093/treephys/tpab177.

Authors

Guoyun Zhang¹, Songfeng Diao¹, Yating Song¹, Caiyun He¹, Jianguo Zhang^{1

2

3}

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, xiangshan street, haidian district, China.
² Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, longpan street, xuanwu district, China.
³ Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

PMID: 34986489
DOI: 10.1093/treephys/tpab177

Abstract

As a new epigenetic mark, DNA N6-adenine (6mA) methylation plays an important role in various biological processes and has been reported in many prokaryotic organisms in recent years. However, the distribution patterns and functions of DNA 6mA modification have been poorly studied in non-model crops. In this study, we observed that the methylation ratio of 6mA was about 0.016% in the sea buckthorn (Hippophae rhamnoides L.) genome using mass spectrometry. We first constructed a comprehensive 6mA landscape in sea buckthorn genome using nanopore sequencing at single-base resolution. Distribution analysis suggested that 6mA methylated sites were widely distributed in the sea buckthorn chromosomes, which were similar to those in Arabidopsis and rice. Furthermore, reduced 6mA DNA methylation is associated with different expression of genes related to the fruit-ripening process in sea buckthorn. Our results revealed that 6mA DNA modification could be considered an important epigenomic mark and contributes to the fruit ripening process in plants.

Keywords: 6mA; epigenetic mark; fruit development; methylation; sea buckthorn.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenine / analysis
Adenine / metabolism
Arabidopsis* / metabolism
DNA / analysis
DNA / metabolism
DNA Methylation
Fruit
Hippophae* / chemistry
Hippophae* / genetics

Substances

DNA
Adenine