Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Orion M Venero; Wakana Sato; Joseph M Heili; Christopher Deich; Katarzyna P Adamala

doi:10.1007/978-1-0716-1998-8_14

Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Methods Mol Biol. 2022:2433:227-235. doi: 10.1007/978-1-0716-1998-8_14.

Authors

Orion M Venero¹, Wakana Sato¹, Joseph M Heili¹, Christopher Deich¹, Katarzyna P Adamala²

Affiliations

¹ Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.
² Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA. kadamala@umn.edu.

PMID: 34985748
DOI: 10.1007/978-1-0716-1998-8_14

Abstract

Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches-with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.

Keywords: 3D-printable; Giant unilamellar vesicles; Liposomal encapsulation; Microfluidics; Synthetic cells.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biomimetics
Emulsions
Liposomes*
Microfluidics* / methods
Printing, Three-Dimensional

Substances

Emulsions
Liposomes