Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms

Erino Araki; Masaya Hane; Rina Hatanaka; Ryota Kimura; Kana Tsuda; Miku Konishi; Naoko Komura; Hiromune Ando; Ken Kitajima; Chihiro Sato

doi:10.1007/s10719-021-10034-8

Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms

Glycoconj J. 2022 Apr;39(2):291-302. doi: 10.1007/s10719-021-10034-8. Epub 2022 Jan 4.

Authors

Erino Araki^{1

2}, Masaya Hane^{1

2

3}, Rina Hatanaka^{1

2}, Ryota Kimura^{1

2}, Kana Tsuda^{1

2}, Miku Konishi⁴, Naoko Komura⁴, Hiromune Ando⁴, Ken Kitajima^{1

2

3}, Chihiro Sato^{5

6

7}

Affiliations

¹ Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
² Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
³ Glyco-BioMedical Research Center (iGMED), Institute for Glyco-core Research (iGCORE), Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
⁴ Institute for Glyco-Core Research (iGCORE), Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
⁵ Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan. chi@agr.nagoya-u.ac.jp.
⁶ Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan. chi@agr.nagoya-u.ac.jp.
⁷ Glyco-BioMedical Research Center (iGMED), Institute for Glyco-core Research (iGCORE), Nagoya University, Chikusa, Nagoya, 464-8601, Japan. chi@agr.nagoya-u.ac.jp.

PMID: 34982351
DOI: 10.1007/s10719-021-10034-8

Abstract

Gangliosides are important components of the membrane and are involved in many biological activities. St8sia5 is an α2,8-sialyltransferase involved in ganglioside synthesis, and has three isoforms. In this study, we analyzed the features of three isoforms, St8sia5-S, -M, and -L that had not been analyzed, and found that only St8sia5-L was localized in the Golgi, while the majority of St8sia5-M and -S were localized in the ER. The localization of Golgi of St8sia5 depended on the stem region. In addition, the incorporation of exogenous GD3 was upregulated only in St8sia5-L expressing cells. Taken together, the localization of St8sia5 is important for the activity of the enzyme.

Keywords: Ganglioside, isoform; Melanoma; Sialic acid; Sialyltransferase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gangliosides* / metabolism
Golgi Apparatus / metabolism
Mice
Protein Isoforms / genetics
Sialyltransferases* / genetics
Sialyltransferases* / metabolism

Substances

Gangliosides
Protein Isoforms
Sialyltransferases