Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC-UV

Liye Wang; Jiangxiu Niu; Pengyuan Wei; Yilei Feng; Mingyue Ding; Chaojun He; Yongjie Ma; Yaolei Zhu; Jia Li; Lingli Huang; Xiya Zhang

doi:10.1016/j.foodchem.2021.131835

Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC-UV

Food Chem. 2022 May 15:376:131835. doi: 10.1016/j.foodchem.2021.131835. Epub 2021 Dec 11.

Authors

Liye Wang¹, Jiangxiu Niu¹, Pengyuan Wei¹, Yilei Feng¹, Mingyue Ding², Chaojun He¹, Yongjie Ma¹, Yaolei Zhu¹, Jia Li¹, Lingli Huang³, Xiya Zhang⁴

Affiliations

¹ College of Food and Drug, Luoyang Normal University, Luoyang, Henan 471934, PR China.
² Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, PR China.
³ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, PR China. Electronic address: huanglingli@mail.hzau.edu.cn.
⁴ Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450002, PR China. Electronic address: zhangxiya@henau.edu.cn.

PMID: 34980530
DOI: 10.1016/j.foodchem.2021.131835

Abstract

To reduce matrix interference and realize simultaneous detection of multiple homologous compounds (trimethoprim (TMP), diaveridine (DVD), ormetoprim (OMP), baquiloprim (BQP), and aditoprim (ADP) in pig, cattle, chicken, and fish muscles), an immunomagnetic bead (IMB)-based sample purification pretreatment with HPLC-UV was developed. A broad-spectrum monoclonal antibody (mAb, named 14C6) was prepared and conjugated with carboxylic-acid-functionalized magnetic nanoparticles using the active ester method to obtain IMBs for sample purification. The extraction solvent was optimized based on the extraction efficiency. Good linearity was observed for all the five analytes (10-200 μg/kg) with the LOD and LOQ of 5 and 10 μg/kg, respectively. The mean recoveries ranged from 62.5% to 76.9%, while the coefficient of variation was <12.2%. The IMB method afforded greater sample purification and enrichment than those achieved with the SPE column-based conventional method. Hence, the IMB-based sample purification is a useful tool to determine 2,4-diaminopyrimidine residues in edible animal tissues.

Keywords: 2,4-Diaminopyrimidines; HPLC–UV; Immunomagnetic separation; Monoclonal antibody; Sample pretreatment.

MeSH terms

Animals
Cattle
Chickens
Chromatography, High Pressure Liquid
Fishes
Food Contamination / analysis*
Meat / analysis*
Pyrimidines / analysis*
Solid Phase Extraction*
Swine
Tandem Mass Spectrometry*

Substances

Pyrimidines
2,4-diaminopyrimidine