Fenton reaction-assisted photodynamic inactivation of calcined melamine sponge against Salmonella and its application

Qiandai Shi; Jing Jing Wang; Lu Chen; Zhiyun Peng; Qiao-Hui Zeng; Yongheng Zhu; Yong Zhao

doi:10.1016/j.foodres.2021.110847

Fenton reaction-assisted photodynamic inactivation of calcined melamine sponge against Salmonella and its application

Food Res Int. 2022 Jan:151:110847. doi: 10.1016/j.foodres.2021.110847. Epub 2021 Dec 2.

Authors

Qiandai Shi¹, Jing Jing Wang², Lu Chen¹, Zhiyun Peng¹, Qiao-Hui Zeng³, Yongheng Zhu¹, Yong Zhao⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Department of Food Science, Foshan University, Foshan 528000, China; Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, Guangdong 350108, China. Electronic address: w_j2010@126.com.
³ Department of Food Science, Foshan University, Foshan 528000, China; Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, Guangdong 350108, China.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China. Electronic address: yzhao@shou.edu.cn.

PMID: 34980385
DOI: 10.1016/j.foodres.2021.110847

Abstract

Photodynamic inactivation (PDI) is an effective alternative to traditional antibiotics to broadly kill bacteria. This study aimed to develop a potent PDI system by coupling calcinated melamine sponges (CMSs) with the Fenton reaction. The results showed that CMS calcined at 350 ℃ was successfully carbonized with intact and porous structures, and it possessed excellent hydrophilicity and photothermal conversion performance. When Fe²⁺ was added and internalized, the Fenton reaction in which Fe²⁺ reacted with H₂O₂ in cells occurred to produce reactive oxygen species (ROS) (OH, OOH, etc.) and O₂, and notably, the O₂ molecules could serve as a raw material to absorb the photothermal energy of CMS to generate highly reactive ¹O₂. Under synergistic effects, CMS-350 coupled with Fe²⁺ potently inactivated > 6 Log CFU/mL (>99.9999%) of Salmonella under 201.6 J/cm² blue LED illumination by destroying Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase, DNA synthesis-related enzymes, cell membranes, etc. Meanwhile, the composite photocatalyst was proven to be nontoxic and could inactivate Salmonella in various foods, including vegetables (Brassica chinensis L), eggs and fresh cucumber juice. As a result, CMS coupled with the Fenton reaction greatly improves the inactivation potency of PDI against harmful bacteria.

Keywords: Calcinated melamine sponges; Fenton reaction; Food; Photocatalysis; Photodynamic inactivation (PDI); Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hydrogen Peroxide*
Reactive Oxygen Species
Salmonella
Triazines*

Substances

Reactive Oxygen Species
Triazines
Hydrogen Peroxide
melamine