Expanded detection range of lateral flow immunoassay endowed with a third-stage amplifier indirect probe

Abstract

Here, a third-stage amplifier indirect probe (TsAIP) based lateral flow immunoassay (LFIA) was proposed to detect furazolidone (FZD) with Prussian blue nanoparticles (PBNPs) as carrier to label the goat anti-mouse antibody-horseradish peroxidase conjugation [GAMA(HRP)]. In this strategy, owing to the fact that one monoclonal antibody (mAb) can combine several GAMA molecules simultaneously, the indirect probe can generate primary signal amplification, then realize second-stage amplification attributing to PBNPs, and finally achieve third-stage amplification because of the conjugated HRP. The TsAIP-based LFIA shows improved performance for FZD metabolite derivative with a detection limit of 1 ng mL^-1. The detection range is expanded about 2-fold compared with the original outcome. Besides, the proposed sensor could be successfully applied in food samples. This method provides a platform for broadening the detection range and application of PBNPs based LFIAs.

Keywords: Indirect probe; Lateral flow immunoassay; Peroxidase-like activity; Prussian blue nanoparticle; Signal amplification.