Protocol for parotidectomy and saliva analysis in mice

Khaled Zubeidat; Yasmin Saba; Or Barel; Fady L Shoukair; Avi-Hai Hovav

doi:10.1016/j.xpro.2021.101048

Protocol for parotidectomy and saliva analysis in mice

STAR Protoc. 2021 Dec 16;3(1):101048. doi: 10.1016/j.xpro.2021.101048. eCollection 2022 Mar 18.

Authors

Khaled Zubeidat¹, Yasmin Saba¹, Or Barel¹, Fady L Shoukair², Avi-Hai Hovav¹

Affiliations

¹ Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
² Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem, Israel.

Abstract

The murine parotid salivary glands develop postnatally, shaping oral mucosal immunity in early and adult life. This protocol details the surgical removal of the parotid glands (parotidectomy) of mice. We also describe a protocol for saliva collection to enable manipulation and measurement of physiological and immunological salivary functions. Our saliva collection approach has been modified from published protocols to enable saliva collection from young mice, which can be challenging. For complete details on the use and execution of this protocol, please refer to Koren et al. (2020).

Keywords: Immunology; Microbiology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mice
Parotid Gland / surgery
Saliva*
Salivary Glands* / surgery