Detection of CD8+ T cell-mediated immune responses to bacterial infection in mice

Jun Wu; Gen Li; Dan Li; Jiawen Zhou; Zhongjun Dong; Peng Jiang

doi:10.1016/j.xpro.2021.101022

Detection of CD8⁺ T cell-mediated immune responses to bacterial infection in mice

STAR Protoc. 2021 Dec 13;2(4):101022. doi: 10.1016/j.xpro.2021.101022. eCollection 2021 Dec 17.

Authors

Jun Wu^{1

2}, Gen Li^{1

2}, Dan Li³, Jiawen Zhou^{1

2}, Zhongjun Dong³, Peng Jiang^{1

2}

Affiliations

¹ School of Life Sciences, Tsinghua University, Beijing 100084, China.
² Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
³ School of Medicine and Institute for Immunology, Tsinghua University, Beijing 100084, China.

Abstract

Efficient activation of CD8⁺ T cells is critical for bacterial resistance and eradicating malignancy in the body. Here, we present a step-by-step protocol to use Listeria monocytogenes expressing OVA (LmOVA) to stimulate endogenous CD8⁺ T cells. We describe the steps for adoptive transfer of OT-I CD8⁺ T cells to CD45.1 mice and then detail the steps for detection of the antigen-specific CD8⁺ T cells in response to LmOVA. For complete details on the use and execution of this protocol, please refer to Wu et al. (2021).

Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Immunology; Microbiology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adoptive Transfer*
Animals
CD8-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / transplantation
Disease Models, Animal
Listeria monocytogenes / immunology
Listeriosis / immunology*
Male
Mice
Mice, Inbred C57BL