The relationship between glycine levels in collagen in the anterior rectus sheath tissue and the onset of indirect inguinal hernia: A cross-sectional study

Amir Fajar; Warsinggih; Erwin Syarifuddin; Joko Hendarto; Ibrahim Labeda; Ronald Erasio Lusikooy; Mappincara; Muhammad Iwan Dani; Samuel Sampetoding; Muhammad Ihwan Kusuma; Julianus Aboyaman Uwuratuw; Muhammad Faruk

doi:10.1016/j.amsu.2021.103166

The relationship between glycine levels in collagen in the anterior rectus sheath tissue and the onset of indirect inguinal hernia: A cross-sectional study

Ann Med Surg (Lond). 2021 Dec 9:73:103166. doi: 10.1016/j.amsu.2021.103166. eCollection 2022 Jan.

Authors

Amir Fajar¹, Warsinggih^{1

2}, Erwin Syarifuddin¹, Joko Hendarto³, Ibrahim Labeda^{1

2}, Ronald Erasio Lusikooy^{1

2}, Mappincara¹, Muhammad Iwan Dani¹, Samuel Sampetoding^{1

2}, Muhammad Ihwan Kusuma^{1

2}, Julianus Aboyaman Uwuratuw¹, Muhammad Faruk⁴

Affiliations

¹ Division of Digestive, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
² Division of Digestive, Department of Surgery, Dr. Wahidin Sudirohusodo General Hospital, Makassar, Indonesia.
³ Department of Biostatistics, Faculty of Public Health, Hasanuddin University, Makassar, Indonesia.
⁴ Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.

Abstract

Introduction: An inguinal hernia is a protrusion of abdominal-cavity contents through the inguinal canal. Protection against an inguinal hernia depends on the integrity of fascial tissue, which is maintained by collagen. Collagen is a structural protein consisting of amino acids, the most common of which is glycine. This study aimed to determine the relationship between glycine and the appearance of lateral inguinal hernias. To this end, the researchers examined the profile of collagen and glycine levels in the tissue of the sheath of the rectus femoris in patients with lateral inguinal hernia (indirect inguinal hernia).

Methods: The study used a cross-sectional design to determine glycine levels in rectus anterior sheath tissue in patients with indirect inguinal hernia. Examination of collagen glycine levels was conducted using the ELISA (Enzyme-Linked immunosorbent assay) method. Data were analyzed using the Statistical Package for the Social Science (SPSS) program. An ANOVA test, Pearson's correlation test, and Spearman's correlation test were also performed. A p-value <0.05 was said to be significant.

Results: Across 72 samples, the mean body mass index (BMI) was 22.5 kg/m2 and, the mean clinical onset was 28.02 months. Correlation tests showed a correlation between glycine levels and clinical onset (p = 0.026). The ANOVA test showed a difference between glycine levels with age group (p = 0.025) and BMI (p = 0.015). The correlation between glycine levels and clinical-grade (p = 0.416) was not statistically significant.

Conclusion: There is a significant relationship between glycine levels and age, BMI, and clinical onset of indirect inguinal hernia.

Keywords: Collagen; Cross-sectional study; ELISA assay; Glycine; Inguinal hernia.