Integrative analysis of transcriptome and proteome provides insights into adaptation to cadmium stress in Sedum plumbizincicola

Yue Zhu; Wenmin Qiu; Xiaoyang He; Longhua Wu; De Bi; Zhiping Deng; Zhengquan He; Chao Wu; Renying Zhuo

doi:10.1016/j.ecoenv.2021.113149

Integrative analysis of transcriptome and proteome provides insights into adaptation to cadmium stress in Sedum plumbizincicola

Ecotoxicol Environ Saf. 2022 Jan 15:230:113149. doi: 10.1016/j.ecoenv.2021.113149. Epub 2021 Dec 30.

Authors

Yue Zhu¹, Wenmin Qiu¹, Xiaoyang He², Longhua Wu³, De Bi⁴, Zhiping Deng⁵, Zhengquan He⁶, Chao Wu⁷, Renying Zhuo⁸

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, PR China; Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, PR China.
² Agricultural Technology Extension Centre of Dongtai, Jiangsu 224200, PR China.
³ Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China.
⁴ Suzhou Polytechnic Institute of Agriculture, Suzhou 215000, PR China.
⁵ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China.
⁶ Key Laboratory of Three Gorges Regional Plant Genetic ＆ Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University, Yichang, 443002 Hubei, PR China. Electronic address: zhq_he@163.com.
⁷ Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, PR China. Electronic address: semporna@126.com.
⁸ State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, PR China; Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang 311400, PR China. Electronic address: zhuory@gmail.com.

PMID: 34974361
DOI: 10.1016/j.ecoenv.2021.113149

Abstract

Sedum plumbizincicola, a cadmium (Cd) hyperaccumulating herbaceous plant, can accumulate large amounts of Cd in the above-ground tissues without being poisoned. However, the molecular mechanisms regulating the processes are not fully understood. In this study, Transcriptional and proteomic analyses were integrated to investigate the response of S. plumbizincicola plants to Cd stress and to identify key pathways that are potentially responsible for Cd tolerance and accumulation. A total of 630 DAPs (differentially abundant proteins, using fold change >1.5 and adjusted p-value <0.05) were identified from Tandem Mass Tag (TMT)- based quantitative proteomic profiling, which were enriched in processes including phenylpropanoid biosynthesis, protein processing in endoplasmic reticulum, and biosynthesis of secondary metabolites. Combined with the previous transcriptomic study, 209 genes and their corresponding proteins showed the identical expression pattern. The identified genes/proteins revealed the potential roles of several metabolism pathways, including phenylpropanoid biosynthesis, oxidative phosphorylation, phagosome, and glutathione metabolism, in mediating Cd tolerance and accumulation. Lignin staining and Cd accumulation assay of the transgenic lines over-expressing a selected Cd up-regulated gene SpFAOMT (Flavonoid 3',5'-methyltransferase) showed its functions in adapting to Cd stress, and provided insight into its role in lignin biosynthesis and Cd accumulation in S. plumbizincicola during Cd stress.

Keywords: Cadmium; Integrative analysis, FAOMT; Proteome; Sedum plumbizincicola; Transcriptome.