A cysteine and Hg2+ detection method based on transformation supramolecular assembly of cyanine dye by AGRO100

Lu Liu; Lei Shi; Jian-Yong Liu; Da-Wei Yang; Yao Fu; Xiao-Ying Ma; Bu-Yue Zhang; Xiu-Feng Zhang

doi:10.1016/j.saa.2021.120779

A cysteine and Hg²⁺ detection method based on transformation supramolecular assembly of cyanine dye by AGRO100

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Apr 5:270:120779. doi: 10.1016/j.saa.2021.120779. Epub 2021 Dec 21.

Authors

Lu Liu¹, Lei Shi², Jian-Yong Liu³, Da-Wei Yang⁴, Yao Fu⁵, Xiao-Ying Ma⁶, Bu-Yue Zhang⁷, Xiu-Feng Zhang⁸

Affiliations

¹ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: 15175570931@sina.cn.
² College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: slbuct@sina.com.
³ State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China. Electronic address: beam@dicp.ac.cn.
⁴ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: yangdwgy@163.com.
⁵ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: yaofu1995@126.com.
⁶ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: xiaoyingma98@163.com.
⁷ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: byzhang98@163.com.
⁸ College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: xfzhang@iccas.ac.cn.

PMID: 34974293
DOI: 10.1016/j.saa.2021.120779

Abstract

ETC (3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-ethylthiacarbocyanine triethylammonium salt), as a derivative of thiazole, is capable of forming various aggregates by the short-range noncovalent interaction forces under specific conditions, accompanying with significant absorbance and fluorescence characteristics. In this work, a label-free probe (ETC) for the detection of Cys (Cysteine) and Hg²⁺ was developed based on transformation between monomers and J-aggregations by AGRO100. AGRO100 can transform between single-stranded DNA and G-quadruplex to realize recognition of Cys and Hg²⁺ in dual-channel mode. These recognitional signals can be captured by UV-visible absorption spectra and fluorescence spectroscopy. ETC exhibits high sensitivity and selectivity with the detection limit of 0.197 nM in a wide range of 0-15 μM, which can apply of Cys and Hg²⁺ detection in human serum.

Keywords: Cyanine dye; Cysteine; G-quadruplex; Hg(2+).

MeSH terms

Aptamers, Nucleotide*
Cysteine
Fluorescent Dyes
Humans
Mercury*
Oligodeoxyribonucleotides

Substances

AGRO 100
Aptamers, Nucleotide
Fluorescent Dyes
Oligodeoxyribonucleotides
Mercury
Cysteine