Studying Autophagy In Vivo in the Mammary Gland and in Xenograft Samples

Abstract

Autophagy is a dynamic process that can be monitored in multiple ways, both in vitro and in vivo. Studies in mice are a widely used tool to understand multiple diseases and conditions where autophagy plays a role, and therefore autophagic flux measurement in tissues of rodent models are of utmost importance. Here, we present some assays successfully used in determining the autophagy status in the mice mammary gland as well as in xenografts.

Keywords: Autophagy; Electron microscopy; GFP-LC3; Immunohistochemistry; Immunoprecipitation; Mammary gland; RNAseq; Western blot; Xenografts.