Using Microinjection of Mammalian Cultured Cells to Study Cell Division

Charles Day; Alyssa Langfald; Edward H Hinchcliffe

doi:10.1007/978-1-0716-1904-9_8

Using Microinjection of Mammalian Cultured Cells to Study Cell Division

Methods Mol Biol. 2022:2415:105-122. doi: 10.1007/978-1-0716-1904-9_8.

Authors

Charles Day^{1

2}, Alyssa Langfald¹, Edward H Hinchcliffe^{3

4}

Affiliations

¹ The Hormel Institute, University of Minnesota, Austin, MN, USA.
² Neuro-Oncology Training Program, Mayo Clinic, Rochester, MN, USA.
³ The Hormel Institute, University of Minnesota, Austin, MN, USA. hinc0002@umn.edu.
⁴ Masonic Cancer Center, University of Minnesota, Austin, MN, USA. hinc0002@umn.edu.

PMID: 34972949
DOI: 10.1007/978-1-0716-1904-9_8

Abstract

The introduction of macromolecules directly into individual cells by microinjection is an important technique for manipulating mitotic cells. mRNA, purified proteins, or concentrated antibodies can all be injected directly into a single cell, and their effects monitored by live-cell imaging. The equipment necessary is relatively simple, and the technique can be easily mastered. Here we describe our microinjection setup, how to microinject cultured mammalian cells in mitosis, and how to analyze those cells by same-cell live and fixed imaging.

Keywords: Antibody; Cell cycle; Fluorescence; Injector; Micromanipulator; Microneedle; Microscopy; Mitosis; Phosphorylation.

MeSH terms

Animals
Cell Line
Cells, Cultured
Mammals*
Microinjections / methods
Mitosis*