Inhibitory activities of phloroglucinol-chitosan nanoparticles on mono- and dual-species biofilms of Candida albicans and bacteria

Fazlurrahman Khan; Dokyung Oh; Pathum Chandika; Du-Min Jo; Nilushi Indika Bamunarachchi; Won-Kyo Jung; Young-Mog Kim

doi:10.1016/j.colsurfb.2021.112307

Inhibitory activities of phloroglucinol-chitosan nanoparticles on mono- and dual-species biofilms of Candida albicans and bacteria

Colloids Surf B Biointerfaces. 2022 Mar:211:112307. doi: 10.1016/j.colsurfb.2021.112307. Epub 2021 Dec 24.

Authors

Fazlurrahman Khan¹, Dokyung Oh², Pathum Chandika³, Du-Min Jo², Nilushi Indika Bamunarachchi², Won-Kyo Jung⁴, Young-Mog Kim⁵

Affiliations

¹ Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
² Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
³ Department of Biomedical Engineering, and New-senior Healthcare Innovation Center (BK21 Plus) Pukyong National University, Busan 48513, Republic of Korea.
⁴ Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Department of Biomedical Engineering, and New-senior Healthcare Innovation Center (BK21 Plus) Pukyong National University, Busan 48513, Republic of Korea.
⁵ Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: ymkim@pknu.ac.kr.

PMID: 34971906
DOI: 10.1016/j.colsurfb.2021.112307

Abstract

Phloroglucinol (PG) was encapsulated into chitosan nanoparticles (CSNPs) using a simple ionic gelification technique, and the inhibitory activity of the resulting nanoparticles on microbial mono- and dual-species biofilms was investigated. PG-CSNPs were determined to be spherical with a rough surface, and had an average diameter and zeta potential of 414.0 ± 48.5 nm and 21.1 ± 1.2 mV, respectively. The rate of PG release from the loaded CSNPs was found to increase in acidic environment. The loading capacity and encapsulation efficiency of PG to CSNPs were determined to be 18.74% and 22.4%, respectively. The prepared PG-CSNPs exhibited inhibitory effects on mono-species biofilms such as Candida albicans, Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus mutans, and dual-species such as C. albicans-K. pneumoniae/S. aureus/S. mutans. The PG-CSNPs were found to be more effective in inhibiting and eradicating mono- and dual-species biofilms than pure PG. In addition, PG-CSNPs were found to enhance the efficacy of several antimicrobial drugs against mature mono- and dual-species biofilms. This work demonstrates that PG-CSNPs may provide an alternative method for treating infections caused by biofilm-forming pathogens.

Keywords: Antibiofilm; Candida albicans; Chitosan; Dual-species biofilms; Nanoparticles; Phloroglucinol.

MeSH terms

Biofilms
Candida albicans
Chitosan* / pharmacology
Nanoparticles*
Phloroglucinol / pharmacology
Staphylococcus aureus

Substances

Chitosan
Phloroglucinol