Highly sensitive and quantitative biodetection with lipid-polymer hybrid nanoparticles having organic room-temperature phosphorescence

Do Hyun Kang; Yingying Zeng; Muneesh Tewari; Jinsang Kim

doi:10.1016/j.bios.2021.113889

Highly sensitive and quantitative biodetection with lipid-polymer hybrid nanoparticles having organic room-temperature phosphorescence

Biosens Bioelectron. 2022 Mar 1:199:113889. doi: 10.1016/j.bios.2021.113889. Epub 2021 Dec 17.

Authors

Do Hyun Kang¹, Yingying Zeng², Muneesh Tewari³, Jinsang Kim⁴

Affiliations

¹ Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.
² Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.
³ Department of Internal Medicine, Hematology/Oncology Division, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
⁴ Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA. Electronic address: jinsang@umich.edu.

PMID: 34968954
DOI: 10.1016/j.bios.2021.113889

Abstract

A versatile organic room-temperature phosphorescence (RTP)-based "turn on" biosensor platform has been devised with high sensitivity by combining oxygen-sensitive lipid-polymer hybrid RTP nanoparticles with a signal-amplifying enzymatic oxygen scavenging reaction in aqueous solutions. When integrated with a sandwich-DNA hybridization assay on 96-well plates, our phosphorimetric sensor demonstrates sequence-specific detection of a cell-free cancer biomarker, a TP53 gene fragment, with a sub-picomolar (0.5 p.m.) detection limit. This assay is compatible with detecting cell-free nucleic acids in human urine samples. Simply by re-programming the detection probe, our unique methodology can be adapted to a broad range of biosensor applications for biomarkers of great clinical importance but difficult to detect due to their low abundance in vivo.

Keywords: Cell-free DNA detection; Enzymatic signal amplification; Lipid-polymer hybrid nanoparticles; Phosphorimetric assay; Purely organic room-temperature phosphorescent nanosensor.

MeSH terms

Biosensing Techniques*
Humans
Lipids
Nanoparticles*
Polymers
Temperature

Substances

Lipids
Polymers