Prussian blue nanoparticles-enabled sensitive and accurate ratiometric fluorescence immunoassay for histamine

Cai-Xia Liao; Bao-Zhu Jia; Hong Wang; Yuan-Ming Sun; Xiao-Yan Xu; Xiao-Qun Wei; Yu-Dong Shen; Hong-Tao Lei; Zhen-Lin Xu; Lin Luo

doi:10.1016/j.foodchem.2021.131907

Prussian blue nanoparticles-enabled sensitive and accurate ratiometric fluorescence immunoassay for histamine

Food Chem. 2021 Dec 22:376:131907. doi: 10.1016/j.foodchem.2021.131907. Online ahead of print.

Authors

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China.
² College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China.
³ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: jallent@163.com.
⁴ Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: lin.luo@scau.edu.cn.

PMID: 34968915
DOI: 10.1016/j.foodchem.2021.131907

Abstract

Herein, a nanozyme-mediated ratiometric fluorescence immunoassay for histamine (HA) has been developed. Prussian blue nanoparticles (PBNPs) with outstanding peroxidase-like activity were labelled with goat anti-mouse IgG via a facile electrostatic adsorption to yield the nanozyme-antibody conjugate which acted as a bridge to link the ratiometric fluorescence readout with HA concentration. As substrate, o-phenylenediamine (OPD) was oxidized into 2,3-diaminophenazine (oxOPD) by H₂O₂ under the catalysis of PBNPs, producing a novel emission at 570 nm and quenching the fluorescence of carbon dots (CDs) at 450 nm simultaneously. Under optimal conditions, the ratio of fluorescence intensity at 570 nm and 450 nm (I₅₇₀/I₄₅₀) linearly correlated with HA concentration ranging from 1.6 ng/mL to 125 μg/mL, with a detection limit (LOD) of 1.2 ng/mL. In addition, analytical performances including specificity, accuracy and applicability were evaluated, which revealed that this ratiometric fluorescence immunoassay affords an effective platform for sensitive and accurate detection of HA.

Keywords: Histamine; Nanozyme; Prussian blue nanoparticles; Ratiometric fluorescence immunoassay.