Proteomic and physiological analysis provides an elucidation of Fusarium proliferatum infection causing crown rot on banana fruit

Lihong Xie; Yanfei Wu; Xuewu Duan; Taotao Li; Yueming Jiang

doi:10.1016/j.micres.2021.126952

Proteomic and physiological analysis provides an elucidation of Fusarium proliferatum infection causing crown rot on banana fruit

Microbiol Res. 2022 Mar:256:126952. doi: 10.1016/j.micres.2021.126952. Epub 2021 Dec 21.

Authors

Lihong Xie¹, Yanfei Wu¹, Xuewu Duan², Taotao Li³, Yueming Jiang⁴

Affiliations

¹ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100039, China.
² Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
³ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address: taotaoli@scbg.ac.cn.
⁴ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address: ymjiang@scbg.ac.cn.

PMID: 34968824
DOI: 10.1016/j.micres.2021.126952

Abstract

Fusarium proliferatum causes the crown rot of harvested banana fruit but the underling infection mechanism remains unclear. Here, proteomic changes of the banana peel with and without inoculation of F. proliferatum were evaluated. In addition, we investigated the effects of F. proliferatum infection on cell structure, hormone content, primary metabolites and defense-related enzyme activities in the banana peel. Our results showed that F. proliferatum infection mainly affects cell wall components and inhibits the activities of polyphenoloxidase, peroxidase, and chitinase. Gel free quantitative proteomic analysis showed 92 down-regulated and 29 up-regulated proteins of banana peel after F. proliferatum infection. These proteins were mainly related to defense response to biotic stress, chloroplast structure and function, JA signaling pathway, and primary metabolism. Although jasmonic acid (JA) content and JA signaling component coronatine-insensitive (COI) protein were induced by F. proliferatum infection, JA-responsible defense genes/proteins were downregulated. In contrast, expression of senescence-related genes was induced by F. proliferatum, indicating that F. proliferatum modulated the JA signaling to accelerate the senescence of banana fruit. Additionally, salicylic acid (SA) content and SA signaling for resistance acquisition were inhibited by F. proliferatum. Taken together, these results suggest that F. proliferatum depolymerizes the cell wall barrier, impairs the defense system in banana fruit, and activates non-defensive JA-signaling pathway accelerated the senescence of banana fruit. This study provided the elucidation of the prominent pathways disturbed by F. proliferatum in banana fruit, which will facilitate the development of a new strategy to control crown rot of banana fruit and improvement of banana cultivars.

Keywords: Enzyme activity; Fruit; Fungal infection; Fusarium; Phytohormone; Proteome.

MeSH terms

Fruit
Fusarium*
Musa*
Proteomics

Supplementary concepts

Fusarium proliferatum