Association between Escherichia coli with NotI-restriction resistance and urinary tract infections

Masayuki Hashimoto; Bin-Hsu Mao; Chien-Shun Chiou; Wen-Chun Huang; Ida Bagus Nyoman Putra Dwija; Shuen-Lin Jeng; Jiunn-Jong Wu; Ming-Cheng Wang; Wei-Hung Lin; Chin-Chung Tseng; Ching-Hao Teng

doi:10.1016/j.jmii.2021.11.010

Association between Escherichia coli with NotI-restriction resistance and urinary tract infections

J Microbiol Immunol Infect. 2022 Aug;55(4):686-694. doi: 10.1016/j.jmii.2021.11.010. Epub 2021 Dec 16.

Authors

Affiliations

¹ Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.
² Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
³ The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City, Taiwan.
⁴ Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁵ Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Microbiology Clinic Department, Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia.
⁶ Department of Statistics, Institute of Data Science, and Center for Innovative FinTech Business Models, National Cheng Kung University, Tainan, Taiwan.
⁷ Department of Biotechnology and Laboratory Science in Medicine, School of Biomedical Science and Engineering, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
⁸ Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City, Taiwan; Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
⁹ Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City, Taiwan.
¹⁰ Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan. Electronic address: chteng@mail.ncku.edu.tw.

PMID: 34963576
DOI: 10.1016/j.jmii.2021.11.010

Abstract

Background: Escherichia coli is the most common cause of urinary tract infections (UTIs). It is widely accepted that uropathogenic E. coli (UPEC) mainly emerge from the distal gut microbiota. Identification of bacterial characteristics that are able to differentiate UPEC from fecal commensal strains will facilitate the development of novel strategies to detect and monitor the spread of UPEC.

Methods: Fifty fecal commensal, 83 UTI-associated and 40 biliary tract infection (BTI)-associated E. coli isolates were analyzed. The NotI restriction patterns of chromosomal DNA in the isolates were determined by pulse-field gel electrophoresis. The phylogenetic types and the presence of 9 known virulence genes of each isolate were determined by PCR analyses. Additionally, the susceptibilities of the isolates to antibiotics were revealed. Then the associations of NotI resistance with UTI-associated isolates, phylotypes, and antibiotic resistance were assessed.

Results: NotI resistance was correlated with UTI-associated isolates, compared to the fecal isolates. Consistently, NotI-resistant isolates harbored a greater number of virulence factors and mainly belonged to phylotype B2. Additionally NotI resistance was correlated with chloramphenicol resistance among the bacteria. Among the fecal, UTI-associated and BTI-associated groups, the distribution of NotI-resistant group B2 isolates was correlated with UTI-associated bacteria.

Conclusion: NotI resistance alone is a potential marker for distinguishing fecal strains and UPEC, while the combination of NotI resistance and B2 phylogeny is a candidate marker to differentiate UPEC from fecal and other extraintestinal pathogenic E. coli. Additionally, NotI resistance may be valuable for assessing the potential of chloramphenicol resistance of E. coli.

Keywords: NotI; PFGE; Phylogenetic group B2; RFLP; Restriction enzyme resistance; Urinary tract infection; Uropathogenic E. coli.

MeSH terms

Anti-Bacterial Agents
Escherichia coli Infections*
Humans
Phylogeny
Urinary Tract Infections*
Uropathogenic Escherichia coli*
Virulence Factors

Substances

Anti-Bacterial Agents
Virulence Factors