Investigation of P1/HC-Pro-Mediated ABA/Calcium Signaling Responses via Gene Silencing through High- and Low-Throughput RNA-seq Approaches

Yen-Hsin Chiu; Yu-Ling Hung; Hsin-Ping Wang; Wei-Lun Wei; Qian-Wen Shang; Thanh Ha Pham; Chien-Kang Huang; Zhao-Jun Pan; Shih-Shun Lin

doi:10.3390/v13122349

Investigation of P1/HC-Pro-Mediated ABA/Calcium Signaling Responses via Gene Silencing through High- and Low-Throughput RNA-seq Approaches

Viruses. 2021 Nov 23;13(12):2349. doi: 10.3390/v13122349.

Authors

Yen-Hsin Chiu^{1

2}, Yu-Ling Hung³, Hsin-Ping Wang¹, Wei-Lun Wei¹, Qian-Wen Shang¹, Thanh Ha Pham¹, Chien-Kang Huang⁴, Zhao-Jun Pan¹, Shih-Shun Lin^{1

5

6}

Affiliations

¹ Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan.
² Seed Improvement and Propagation Station, Council of Agriculture, Taichung 427, Taiwan.
³ Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
⁴ Department of Engineering Science and Ocean Engineering, National Taiwan University, Taipei 106, Taiwan.
⁵ Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.
⁶ Center of Biotechnology, National Taiwan University, Taipei 106, Taiwan.

Abstract

The P1/HC-Pro viral suppressor of potyvirus suppresses posttranscriptional gene silencing (PTGS). The fusion protein of P1/HC-Pro can be cleaved into P1 and HC-Pro through the P1 self-cleavage activity, and P1 is necessary and sufficient to enhance PTGS suppression of HC-Pro. To address the modulation of gene regulatory relationships induced by turnip mosaic virus (TuMV) P1/HC-Pro (P1/HC-Pro^Tu), a comparative transcriptome analysis of three types of transgenic plants (P1^Tu, HC-Pro^Tu, and P1/HC-Pro^Tu) were conducted using both high-throughput (HTP) and low-throughput (LTP) RNA-Seq strategies. The results showed that P1/HC-Pro^Tu disturbed the endogenous abscisic acid (ABA) accumulation and genes in the signaling pathway. Additionally, the integrated responses of stress-related genes, in particular to drought stress, cold stress, senescence, and stomatal dynamics, altered the expressions by the ABA/calcium signaling. Crosstalk among the ABA, jasmonic acid, and salicylic acid pathways might simultaneously modulate the stress responses triggered by P1/HC-Pro^Tu. Furthermore, the LTP network analysis revealed crucial genes in common with those identified by the HTP network in this study, demonstrating the effectiveness of the miniaturization of the HTP profile. Overall, our findings indicate that P1/HC-Pro^Tu-mediated suppression in RNA silencing altered the ABA/calcium signaling and a wide range of stress responses.

Keywords: ABA signaling; HTP-Seq; LTP-Seq; P1/HC-ProTu; calcium signaling; stress response.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis* / genetics
Arabidopsis* / virology
Calcium Signaling / genetics*
Gene Expression Regulation, Plant
Plants, Genetically Modified / virology*
RNA Interference