The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines. Part I. Isolation, Identification and In Vitro Studies

Csilla Kovács; András Csótó; Károly Pál; Antal Nagy; Erzsébet Fekete; Levente Karaffa; Christian P Kubicek; Erzsébet Sándor

doi:10.3390/pathogens10121612

The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines. Part I. Isolation, Identification and In Vitro Studies

Pathogens. 2021 Dec 10;10(12):1612. doi: 10.3390/pathogens10121612.

Authors

Csilla Kovács¹, András Csótó^{2

3}, Károly Pál⁴, Antal Nagy², Erzsébet Fekete⁵, Levente Karaffa^{5

6}, Christian P Kubicek⁷, Erzsébet Sándor⁴

Affiliations

¹ Research Institute Újfehértó, Agricultural Research and Educational Farm, University of Debrecen, Vadas tag 2, H-4244 Újfehértó, Hungary.
² Institute of Plant Protection, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi út 138, H-4032 Debrecen, Hungary.
³ Kálmán Kerpely Doctoral School, University of Debrecen, Böszörményi út 138, H-4032 Debrecen, Hungary.
⁴ Institute of Food Science, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, Böszörményi út 138, H-4032 Debrecen, Hungary.
⁵ Department of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary.
⁶ Institute of Metagenomics, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary.
⁷ Institute of Chemical, Environmental & Bioscience Engineering, TU Wien, Vienna A-1060, Austria.

Abstract

This paper reports on the identification and in vitro characterization of several Trichoderma strains isolated from the Tokaj Wine Region in North-East Hungary. Ten isolates were analyzed and found to consist of six individual species-T. gamsii, T. orientale, T. simmonsii, T. afroharzianum, T. atrobrunneum and T. harzianum sensu stricto. The growth potential of the strains was assessed at a range of temperatures. We also report here on the in vitro biocontrol properties and fungicide tolerance of the most promising strains.

Keywords: Trichoderma afroharzianum; Trichoderma simmonsii; Vitis vinifera; dual plate tests; fungicide tolerance; mycelial growth.

Abstract

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