Adsorption, and controlled release of doxorubicin from cellulose acetate/polyurethane/multi-walled carbon nanotubes composite nanofibers

Reza Alisani; Navid Rakhshani; Maryam Abolhallaj; Foojan Motevalli; Parvaneh Ghaderi-Shekhi Abadi; Mohammad Akrami; Mohsen Shahrousvand; Fariborz Sharifian Jazi; Mohammad Irani

doi:10.1088/1361-6528/ac467b

Adsorption, and controlled release of doxorubicin from cellulose acetate/polyurethane/multi-walled carbon nanotubes composite nanofibers

Nanotechnology. 2022 Jan 19;33(15). doi: 10.1088/1361-6528/ac467b.

Authors

Reza Alisani¹, Navid Rakhshani², Maryam Abolhallaj³, Foojan Motevalli³, Parvaneh Ghaderi-Shekhi Abadi⁴, Mohammad Akrami⁵, Mohsen Shahrousvand⁶, Fariborz Sharifian Jazi⁷, Mohammad Irani⁵

Affiliations

¹ Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
² Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
³ Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
⁴ Department of Pharmaceutics, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.
⁵ Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
⁶ Caspian Faculty of Engineering, College of Engineering, Chooka Branch, University of Tehran, PO Box 119-43841, 4386156387, Rezvanshahr Guilan Province, Iran.
⁷ Mining and Metallurgical Engineering Department, Amirkabir University of Technology, Tehran, Iran.

PMID: 34959231
DOI: 10.1088/1361-6528/ac467b

Abstract

The cellulose acetate (CA)/poly (ε-caprolactone diol)/poly (tetramethylene ether) glycol-polyurethane (PCL-Diol/PTMG-PU)/multi-walled carbon nanotubes (MWCNTs) composite nanofibers were prepared via two-nozzle electrospinning on both counter sides of the collector. The performance of synthesized composite nanofibers was investigated as an environmental application and anticancer delivery system for the adsorption/release of doxorubicin (DOX). The synergic effect of MWCNTs and DOX incorporated into the nanofibers was investigated against LNCaP prostate cancer cells. The status of MWCNTs and DOX in composite nanofibers was demonstrated by SEM, FTIR and UV-vis determinations. The adsorption tests using nanofibrous adsorbent toward DOX sorption was evaluated under various DOX initial concentrations (100-2000 mg l^-1), adsorption times (5-120 min), and pH values (pH:2-9). Due to the fitting of isotherm and kinetic data with Redlich-Peterson and pseudo-second order models, both chemisorption and surface adsorption of DOX molecules mechanisms have been predicted. The drug release from both nanofibers and MWCNTs-loaded nanofibers was compared. The better drug sustained release profiles verified in the presence of composite nanofibers. LNCaP prostate cancer and L929 normal cells were treated to investigate the cytotoxicity and compatibility of synthesized composite nanofibers. The apoptosis/necrosis of hybrid nanofibers and MWCNTs loaded-nanofibers was investigated. The obtained results demonstrated the synergic effects of MWCNTs and DOX loaded-nanofibers on the LNCaP prostate cancer cells death.

Keywords: adsorption; cancer; cellulose acetate/multi-walled carbon nanotubes; controlled release.