The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

E N Kashinskaya; E P Simonov; P G Vlasenko; G N Markevich; A V Shokurova; K B Andree; M M Solovyev

doi:10.21307/jofnem-2021-106

The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

J Nematol. 2021 Dec 15:53:e2021-106. doi: 10.21307/jofnem-2021-106. eCollection 2021.

Authors

E N Kashinskaya¹, E P Simonov^{1

2}, P G Vlasenko¹, G N Markevich³, A V Shokurova¹, K B Andree⁴, M M Solovyev^{1

5}

Affiliations

¹ Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobionts; Frunze St. 11, Novosibirsk, 630091, Russia.
² University of Tyumen, Institute of Environmental and Agricultural Biology (X-BIO), 25 Lenina St., Tyumen, 625003, Russia.
³ Kronotsky State Nature Biosphere Reserve, Yelizovo.
⁴ Instituto de Investigación y Tecnología Agroalimentarias; Cultius Aquàtics; San Carlos de la Rapita, Tarragona, ES 08140, Spain.
⁵ Tomsk State University; Institute of Biology, Ecology, Soil Science, Agriculture, and Forestry; 36 Lenin Ave, Tomsk, 634050, Russia.

Abstract

Using the approach of sequencing the V3-V4 region of the 16S rRNA gene, we have analyzed the bacterial diversity associated with the gut and "body" (other parts of nematode after dissection: cuticle, epidermis and longitudinal muscles, etc) of Cystidicola farionis parasitizing the swim bladder of different morphotypes of the nosed charr. Comparisons of the gut microbiota of nematodes with their "body" has revealed that the associated microbiota are closely related to each other. Taxonomic analysis indicated that the relative abundances of the dominant nematode-associated bacteria varied with individual fish. The common dominant microbiota of the gut and "body" of nematodes were represented by Aeromonas, Pseudomonas, Shewanella, and Yersinia, while the associated microbiota of the swim bladder of the nosed charr was dominated by Acinetobacter, Cetobacterium, Pajaroellobacter, Paracoccus, Pseudomonas, Shewanella. By comparing the associated microbiota of nematode parasitizing the different morphotypes of the nosed charr the difference in richness estimates (number of OTU's and Chao1) were revealed between the N1g and N2 morphs.

Keywords: 18S and 28S rRNA; Cystidicola farionis; Gut microbiota; Nosed charr; Salvelinus malma complex; V3–V4 region of 16S rRNA.