High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method

Youjun Zeng; Jie Zhou; Wei Sang; Weifu Kong; Junle Qu; Ho-Pui Ho; Kaiming Zhou; Bruce Zhi Gao; Jiajie Chen; Yonghong Shao

doi:10.3389/fchem.2021.801355

High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method

Front Chem. 2021 Dec 8:9:801355. doi: 10.3389/fchem.2021.801355. eCollection 2021.

Authors

Youjun Zeng¹, Jie Zhou¹, Wei Sang¹, Weifu Kong¹, Junle Qu¹, Ho-Pui Ho², Kaiming Zhou³, Bruce Zhi Gao⁴, Jiajie Chen¹, Yonghong Shao¹

Affiliations

¹ Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, Hong Kong SAR, China.
² Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
³ Department of Bioengineering and COMSET, Clemson University, Clemson, SC, United States.
⁴ Aston Institute of Photonic Technologies, Aston University, Birmingham, United Kingdom.

Abstract

Intensity interrogation surface plasmon resonance (ISPR) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. However, it has relatively low sensitivity, especially for ISPR imaging (ISPRi). In this paper, a new technique for the real-time monitoring of biomolecule binding on sensor surfaces via ISPRi detection is described. The technique is based on the interrogation of the differential value of two intensities at two specific wavelengths from the reflected light spectrum. In addition, we also optimized the selection of dual-wavelength parameters under different circumstances to achieve the highest sensitivity. The new technique achieved a refractive index resolution (RIR) of 2.24 × 10^-6 RIU, which is far beyond that of traditional ISPRi technique. Moreover, our new ISPRi technique also realized the real-time detection of high-throughput biomolecular binding. This study is expected to promote the development of faster and more accurate SPRi technologies.

Keywords: biomolecule interaction; biophotonics and plasmonics; biosensing and bioimaging; surface plasmon; surface plasmon sensors.