A FeS2NPs-Luminol-MnO2NSs system based on chemiluminescence resonance energy transfer platform for sensing glutathione

Abstract

In this work, ferrous disulfide nanoparticles (FeS₂NPs) with oxidase properties were synthesized, and a FeS₂NPs-Luminol-MnO₂ nanosheets (MnO₂NSs) chemiluminescence resonance energy transfer (CRET) system was successfully established. Because of reaction with MnO₂NSs, glutathione (GSH) can inhibit CRET between Luminol and MnO₂NSs and recover the luminescence intensity of FeS₂NPs-Luminol. Consequently, we developed a GSH sensor based on this chemiluminescence resonance energy transfer (CRET) system. Under optimal conditions, the FeS₂NPs-Luminol-MnO₂NSs sensing system showed very sensitive response to GSH in the range of 1 μM-500 μM. The limit of detection of GSH reached as low as 0.15 μM. Finally, the sensor was successfully used for the detection of GSH in serum.

Keywords: Chemiluminescence resonance energy transfer (CRET); Ferrous disulfide nanoparticles (FeS(2)NPs); Glutathione (GSH); Luminol; MnO(2) nanosheets (MnO(2)NSs).