DNA-PKcs kinase activity orchestrates both end-processing and end-ligation

Demis Menolfi; Shan Zha

doi:10.1016/j.tcb.2021.12.002

DNA-PKcs kinase activity orchestrates both end-processing and end-ligation

Trends Cell Biol. 2022 Feb;32(2):91-93. doi: 10.1016/j.tcb.2021.12.002. Epub 2021 Dec 22.

Authors

Demis Menolfi¹, Shan Zha²

Affiliations

¹ Institute for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University, New York City, NY 10032, USA.
² Institute for Cancer Genetics, Vagelos College of Physicians and Surgeons, Columbia University, New York City, NY 10032, USA; Division of Pediatric Oncology, Hematology and Stem Cell Transplantation, Department of Pediatrics, Vagelos College of Physicians and Surgeons, Columbia University, New York City, NY 10032, USA. Electronic address: sz2296@cumc.columbia.edu.

PMID: 34953661
DOI: 10.1016/j.tcb.2021.12.002

Abstract

Recent structural studies have revealed the atomic details of how the DNA-PKcs kinase protects DNA ends, recruits and activates Artemis endonuclease for end-processing, and phosphorylates itself for end-ligation, revealing the molecular details from end-processing to end-ligation, two critical phases of the non-homologous end-joining (NHEJ) DNA-double strand break repair pathway.

Keywords: Artemis; DNA end-ligation; DNA end-processing; DNA-PK holoenzyme; non-homologous end-joining.

MeSH terms

DNA / metabolism
DNA Breaks, Double-Stranded
DNA End-Joining Repair*
DNA Repair
DNA-Binding Proteins* / metabolism
Endonucleases / genetics
Endonucleases / metabolism
Humans

Substances

DNA-Binding Proteins
DNA
Endonucleases

Abstract

MeSH terms

Substances

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