Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p

Wenjing Xiao; Dezhi Zheng; Xin Chen; Botao Yu; Kaiwen Deng; Jie Ma; Xudong Wen; Yonghe Hu; Jun Hou

doi:10.1016/j.isci.2021.103518

Long non-coding RNA MIAT is involved in the regulation of pyroptosis in diabetic cardiomyopathy via targeting miR-214-3p

iScience. 2021 Nov 26;24(12):103518. doi: 10.1016/j.isci.2021.103518. eCollection 2021 Dec 17.

Authors

Wenjing Xiao^{1

2}, Dezhi Zheng³, Xin Chen⁴, Botao Yu², Kaiwen Deng², Jie Ma², Xudong Wen⁵, Yonghe Hu^{1

2}, Jun Hou^{1

2}

Affiliations

¹ School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China.
² Department of Pharmacy, The General Hospital of Western Theater Command of PLA, No 270 Rongdu Road, Jinniu District, Chengdu 610083, China.
³ Department of Cardiovascular Surgery, The 960th Hospital of the PLA Joint Logistic Support Force, Jinan 250031, China.
⁴ Affiliated Hospital of Southwest Jiaotong University & The Third People's Hospital of Chengdu, Chengdu 610015, China.
⁵ Department of Gastroenterology and Hepatology, Chengdu First People's Hospital, Chengdu 610016, China.

Abstract

Diabetic cardiomyopathy (DCM) is one of the most common complications of diabetes without effective treatment options. Its pathogenesis is complex and remains unclear. Long non-coding RNA (lncRNA) MIAT allele has been reported to be enriched in DCM patients and activate a pyroptosis program in hypoxia-induced H9c2 cells. Thus, whether MIAT played a role in DCM pyroptosis remains to be clarified. In the study, the expression of MIAT was found elevated in the serum of diabetic patients, as well as in high-glucose induced cardiomyocytes and diabetic mice. Further, the expression levels of CASP1 and pyroptosis correlation factors (IL-1 and IL-18) were downregulated after silencing MIAT. Through modeling and validation experiments, we then confirmed that the MIAT-miR-214-3p-CASP1 axis serves as an essential point in pyroptosis of DCM mice. These results suggested that silencing MIAT would be a potential treatment strategy for DCM.

Keywords: Cellular physiology; Classification Description: Physiology; Molecular biology.