Arcuate proopiomelanocortin is part of a novel neural connection for short-term low-degree of high ambient temperature effects on food intake

Abstract

High ambient temperature (HTa) is an important environmental factor influencing food intake (FI). We previously demonstrated that low-degree HTa exposure decreased FI earlier than activated physiological responses, and this effect was related to the median preoptic nucleus (MnPO) and arcuate nucleus (Arc) connection. The present study refines the condition of low-degree HTa exposure and focuses on the mechanism of Arc neural activation. We demonstrated in the first experiment that with the usual ambient temperature (Ta) at 23 °C, the low degree HTa condition is at a 7 °C temperature difference and with 90 min exposure. Rats exposed to this short-term low-degree HTa had significantly lower 1-h FI than those exposed to control Ta (CTa) without differences in rectal temperature and hematocrit. Under nonfeeding conditions, HTa could enhance c-Fos at the Arc without the activation of proopiomelanocortin (POMC) neurons. Under feeding conditions, HTa could enhance both c-Fos and POMC at Arc. In addition, the number of c-Fos and POMC colocalizations in the HTa group was higher than that in the CTa group. Finally, intracerebral preinfusion with a subthreshold dose of the melanocortin antagonist SHU9119 reversed the effect of low-degree HTa exposure on FI. Therefore, we conclude that the effect of short-term low-degree HTa exposure on FI in rats is mediated in part by activation of POMC neurons at the Arc. The results partially support the hypothesis that Arc is a crucial hypothalamic nucleus for the effect of low-degree HTa exposure on FI.

Keywords: Ambient temperature; Anorexia; Arcuate nucleus; POMC; Rat.