β-Pinene alleviates arsenic (As)-induced oxidative stress by modulating enzymatic antioxidant activities in roots of Oryza sativa

Abstract

Rice (Oryza sativa L.) is a highly consumed staple crop worldwide, but abiotic/heavy metal stresses acting on the plant cause reduction in yield and quality, thereby impacting global food security. In the present study, we examined the effect of β-pinene against Arsenic (As)-induced oxidative damage vis-à-vis regulation of activities of enzymatic antioxidants in roots of O. sativa. Effect of As (50 μM), β-pinene (10 μM; β-10) and As + β-10 treatments on root length, shoot length, As accumulation, lipid peroxidation (as malondialdehyde [MDA] content), hydrogen peroxide (H₂O₂) accumulation, and activities of lipoxygenase (LOX) and enzymatic antioxidants such as ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) was determined. Exposure of As caused a decline in root and shoot length, and enhancement in As accumulation, lipid peroxidation, and activities of enzymatic antioxidants. However, supplementation of β-10 (i.e., As + β-10 treatments) led to an increase in root and shoot length. Treatment with As + β-10 resulted in a decline in As accumulation, H₂O₂ content, and MDA content; however, the effect on LOX activity was non-significant, as compared to control. Similarly, with As + β-10 treatment a reduction in the activities of APX, GPX, GR, SOD, and CAT was observed as compared with As-alone treatment. Pearson's correlation matrix exhibited strong negative correlation between reactive oxygen species (ROS) and root/shoot length, whereas a strong positive correlation was observed between antioxidant enzymes and ROS. The present study demonstrated that β-pinene significantly ameliorates As-induced oxidative stress and provides tolerance to O. sativa against As-induced toxicity, and thus offer an option of As-mitigation using environment friendly natural plant products. However, to gain insights into the function of β-pinene in modulating As-induced oxidative damage in plants, further field investigations and exploration of its mechanism of action are needed.

Keywords: Arsenic toxicity; Heavy metal stress; Membrane lipid peroxidation; Monoterpene; β-Pinene.