Generation of an iPSC line (ZZUNEUi021-A) from a hypertrophic cardiomyopathy patient with TNNT2 mutation

Yiming Dong; Xiaowei Li; Wanrong Fu; Xiaoxu Tian; Guangli Guo; Ying Peng; Yafei Zhai; Jiangtao Zhao; Zhongren Ding; Xiaoyan Zhao; Jianzeng Dong

doi:10.1016/j.scr.2021.102622

Generation of an iPSC line (ZZUNEUi021-A) from a hypertrophic cardiomyopathy patient with TNNT2 mutation

Stem Cell Res. 2022 Jan:58:102622. doi: 10.1016/j.scr.2021.102622. Epub 2021 Dec 11.

Authors

Affiliations

¹ Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China.
² Centre for Cardiovascular Diseases, Henan Key Laboratory of Hereditary Cardiovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China. Electronic address: jzdong@zzu.edu.cn.

PMID: 34929444
DOI: 10.1016/j.scr.2021.102622

Abstract

A 25-years-old hypertrophic cardiomyopathy male patient donated his peripheral blood mononuclear cells (PBMCs) with heterozygote mutation in theTNNT2 gene. We generated induced pluripotent stem cell (iPSC) with normal karyotypic and expressing NANOG, Lin28, GDF3 and DNMT3. The iPSC line has demonstrated pluripotency by differentiating into three germ layers in vitro. The ZZUNEUi021-A would serve as an in vitro model for loss of TNNT2 function.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cardiomyopathy, Hypertrophic* / genetics
Cell Differentiation
Genes, Homeobox
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male
Mutation / genetics
Troponin T / genetics

Substances

TNNT2 protein, human
Troponin T