Optimization the extraction of anthocyanins from blueberry residue by dual-aqueous phase method and cell damage protection study

Yuan Gao; Yubin Ji; Fuling Wang; Wenlan Li; Xiaomeng Zhang; Zhihui Niu; Zhuo Wang

doi:10.1007/s10068-021-00994-w

Optimization the extraction of anthocyanins from blueberry residue by dual-aqueous phase method and cell damage protection study

Food Sci Biotechnol. 2021 Nov 26;30(13):1709-1719. doi: 10.1007/s10068-021-00994-w. eCollection 2021 Dec.

Authors

Yuan Gao^#^{1

2}, Yubin Ji^#^{1

2}, Fuling Wang¹, Wenlan Li^{1

2}, Xiaomeng Zhang¹, Zhihui Niu¹, Zhuo Wang¹

Affiliations

¹ School of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang 150076 People's Republic of China.
² Post-Doctoral Research Center of Traditional Chinese Medicine, Harbin University of Commerce, Harbin, Heilongjiang 150076 People's Republic of China.

^# Contributed equally.

Abstract

Blueberry residue is usually discarded as waste, but has a high anthocyanins content. The extraction method of anthocyanins from blueberry residue with ultrasonic assisted dual-aqueous phase system was optimized. In terms of the principle of central group and design (CCD) experimental design, three-factor and five-level response surface analysis was adopted to optimize the extraction conditions with the extraction rate of anthocyanins. The optimum extraction rate of anthocyanin was 12.372 ± 0.078 mg/g. Anthocyanin extract could protect the pBR322 DNA oxidative damage induced by Fenton reagent, increase the superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) enzyme activities, and decrease the H₂O₂-induced cell apoptosis of human normal liver cell (LO2 cell). The study indicates that the extraction rate of anthocyanin was increased by optimized ultrasonic assisted dual-aqueous phase system. The anthocyanin extract could protect DNA and LO2 cell from oxidative damage.

Keywords: Anthocyanin; Blueberry residue; Dual-aqueous phase; GSH-Px; SOD.