Role of neuron-derived ATP in paclitaxel-induced HMGB1 release from macrophages and peripheral neuropathy

Risa Domoto; Fumiko Sekiguchi; Riki Kamaguchi; Maiko Iemura; Hiroki Yamanishi; Maho Tsubota; Dengli Wang; Masahiro Nishibori; Atsufumi Kawabata

doi:10.1016/j.jphs.2021.11.003

Role of neuron-derived ATP in paclitaxel-induced HMGB1 release from macrophages and peripheral neuropathy

J Pharmacol Sci. 2022 Jan;148(1):156-161. doi: 10.1016/j.jphs.2021.11.003. Epub 2021 Nov 12.

Authors

Risa Domoto¹, Fumiko Sekiguchi¹, Riki Kamaguchi¹, Maiko Iemura¹, Hiroki Yamanishi¹, Maho Tsubota¹, Dengli Wang², Masahiro Nishibori², Atsufumi Kawabata³

Affiliations

¹ Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan.
² Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
³ Laboratory of Pharmacology and Pathophysiology, Faculty of Pharmacy, Kindai University, Higashi-Osaka 577-8502, Japan. Electronic address: kawabata@phar.kindai.ac.jp.

PMID: 34924121
DOI: 10.1016/j.jphs.2021.11.003

Abstract

We examined the role of ATP and high mobility group box 1 (HMGB1) in paclitaxel-induced peripheral neuropathy (PIPN). PIPN in mice was prevented by HMGB1 neutralization, macrophage depletion, and P2X₇ or P2X₄ blockade. Paclitaxel and ATP synergistically released HMGB1 from macrophage-like RAW264.7 cells, but not neuron-like NG108-15 cells. The paclitaxel-induced HMGB1 release from RAW264.7 cells was accelerated by co-culture with NG108-15 cells in a manner dependent on P2X₇ or P2X₄. Paclitaxel released ATP from NG108-15 cells, but not RAW264.7 cells. Thus, PIPN is considered to involve acceleration of HMGB1 release from macrophages through P2X₇ and P2X₄ activation by neuron-derived ATP.

Keywords: ATP; Chemotherapy-induced peripheral neuropathy (CIPN); High mobility group box 1 (HMGB1); Neuroimmune crosstalk; Paclitaxel.

MeSH terms

Adenosine Triphosphate / physiology*
Animals
HMGB1 Protein / metabolism*
Macrophages / metabolism*
Male
Mice
Mice, Inbred Strains
Neurons / metabolism*
Paclitaxel / adverse effects*
Peripheral Nervous System Diseases / chemically induced*
Peripheral Nervous System Diseases / immunology
Peripheral Nervous System Diseases / metabolism*
Peripheral Nervous System Diseases / prevention & control
RAW 264.7 Cells
Receptor Cross-Talk / immunology
Receptors, Purinergic P2X4 / metabolism
Receptors, Purinergic P2X7 / metabolism

Substances

HMGB1 Protein
HMGB1 protein, mouse
P2rx7 protein, mouse
Receptors, Purinergic P2X4
Receptors, Purinergic P2X7
Adenosine Triphosphate
Paclitaxel