In this study, three bacteria were isolated from activated sludge (Pseudomonas aeruginosa, Bacillus subtilis, and Dietzia maris). After that, isolated strains and Scenedesmus quadricauda that could degrade refractory organics, as co-immobilization species, were prepared gel beads to treat black odorous water. Under the optimized conditions, the removal rate of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), total nitrogen (TN) and total phosphorus (TP) reached 94.36%, 95.7%, 91.22% and 95.27%, respectively, and organics (including aromatic proteins and microbial-by-product-like compounds) were also significantly removed. Microbial analysis reveals that the community structure had a significant difference before and after treatment, and the main dominant at the genus level was transformed from Nitrospirillum (approximately 18.03%) to Flavobacterium (approximately 17.64%). This study also found that the immobilized gel beads have excellent stability and reusability, which provided a feasible and robust bioremediation strategy for the treatment of actual black-odor water.
Keywords: Bioremediation; Black odorous water; Co-immobilized microalgae-bacteria; Isolation.
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