Host-pathogen interactions: The role of Pseudomonas aeruginosa exotoxin A in modulation of Galleria mellonella immune response

Bartłomiej Iwański; Mariola Andrejko

doi:10.1016/j.jip.2021.107706

Host-pathogen interactions: The role of Pseudomonas aeruginosa exotoxin A in modulation of Galleria mellonella immune response

J Invertebr Pathol. 2022 Jan:187:107706. doi: 10.1016/j.jip.2021.107706. Epub 2021 Dec 15.

Authors

Bartłomiej Iwański¹, Mariola Andrejko²

Affiliations

¹ Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., Lublin 20-033, Poland. Electronic address: bartlomiej.iwanski11@wp.pl.
² Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., Lublin 20-033, Poland.

PMID: 34919944
DOI: 10.1016/j.jip.2021.107706

Abstract

The role of Pseudomonas aeruginosa exotoxin A in the modulation of humoral immune response parameters in the hemolymph of Galleria mellonella larvae was investigated. Our results indicate that exoA can play a role of a virulence factor by inhibiting insect PO, lysozyme, and antibacterial activity and decreasing the apoLp-III protein level significantly. No peptide bands with molecular mass below 6.5 kDa were detected in the hemolymph of exoA-treated larvae. We provided evidence for involvement of exoA in the pathogenicity of P. aeruginosa against G. mellonella and the usefulness of the insect as a model for analysis of P. aeruginosa toxins.

Keywords: Galleria mellonella; Host-pathogen interactions; Immune response; Pseudomonas aeruginosa; exotoxin A.

MeSH terms

ADP Ribose Transferases
Animals
Bacterial Toxins
Exotoxins
Hemolymph
Host-Pathogen Interactions
Immunity
Larva
Moths*
Pseudomonas aeruginosa
Pseudomonas aeruginosa Exotoxin A
Virulence Factors

Substances

Bacterial Toxins
Exotoxins
Virulence Factors
ADP Ribose Transferases