Portable quantification of silver ion by using personal glucose meter (PGM) and magnetite cross-linked invertase aggregates (MCLIA)

Abstract

Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the basic analytical methods. In this study, magnetic cross-linked invertase aggregates (MCLIA) were developed for the first time as an enzyme-based signaling platform to detect Ag⁺ using a personal glucose meter (PGM). EDX, FTIR, and VSM results depicted that MCLIA was successfully developed and exhibits super-paramagnetism. In addition, MCLIA selectively detected the Ag⁺ at a sensitivity of 1.2 inhibition rate/μM in a linear range from 5 to 70 μM with a detection limit of 4.6 μM and IC₅₀ value of 42.3 μM. These findings strongly indicate that MCLIA is applicable as a signal platform for portable quantification of other analytes that inhibits the invertase enzyme.

Keywords: Ag(+) detection; Enzyme aggregates; Invertase inhibition; PGM; Portable quantification.