Protocol for sleep analysis in the brain of genetically modified adult mice

Kanako Iwasaki; Noriko Hotta-Hirashima; Hiromasa Funato; Masashi Yanagisawa

doi:10.1016/j.xpro.2021.100982

Protocol for sleep analysis in the brain of genetically modified adult mice

STAR Protoc. 2021 Dec 8;2(4):100982. doi: 10.1016/j.xpro.2021.100982. eCollection 2021 Dec 17.

Authors

Kanako Iwasaki¹, Noriko Hotta-Hirashima^{1

2}, Hiromasa Funato^{1

2}, Masashi Yanagisawa^{1

3

4}

Affiliations

¹ International Institute for Integrative Sleep Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
² Department of Anatomy, Graduate School of Medicine, Toho University, Ota-ku, Tokyo 951-8585, Japan.
³ Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
⁴ Life Science Center, Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.

Abstract

Elucidating the molecular pathways that regulate animal behavior such as sleep is essential for understanding how the brain works. However, to examine how a certain functional domain of protein is involved in animal behavior is challenging. Here, we present a protocol for inducing endogenous protein that lacks a specific functional domain using Cre-mediated allele modification in neurons followed by electroencephalogram/electromyogram (EEG/EMG) recording to study the role of kinases in sleep. This strategy is applicable to other gene targets or behaviors. For complete details on the use and execution of this protocol, please refer to Iwasaki et al. (2021).

Keywords: Behavior; Model Organisms; Molecular Biology; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal / physiology*
Brain* / diagnostic imaging
Brain* / enzymology
Brain* / physiology
Electroencephalography*
Electromyography*
Mice
Mice, Transgenic
Phosphotransferases
Sleep* / genetics
Sleep* / physiology

Substances

Phosphotransferases