Fluorescence determination of lactate dehydrogenase activity based on silicon quantum dots

Yangzhe Zhou; Min Qi; Minghui Yang

doi:10.1016/j.saa.2021.120697

Fluorescence determination of lactate dehydrogenase activity based on silicon quantum dots

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 5:268:120697. doi: 10.1016/j.saa.2021.120697. Epub 2021 Dec 3.

Authors

Yangzhe Zhou¹, Min Qi², Minghui Yang³

Affiliations

¹ Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
² Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China. Electronic address: qimin05@csu.edu.cn.
³ College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: yangminghui@csu.edu.cn.

PMID: 34915230
DOI: 10.1016/j.saa.2021.120697

Abstract

Silicon quantum dots (SiQDs) synthesized based on 3-aminopropyltrimethoxysilane (ATPMS) as silicon source were used to detect the activity of lactate dehydrogenase (LDH) through changes of fluorescence intensity of SiQDs. In this system, the fluorescence of SiQDs was first quenched by nicotinamide adenine dinucleotide (NADH), and then recovered with the addition of LDH, as NADH was consumed by catalytic reaction of LDH. A linear calibration chart of LDH is obtained in the range of 0.77-385 U/mL. The assay displays high selectivity towards LDH detection, and was successfully applied to the analysis of LDH in human serum samples. This assay has great prospects for the diagnosis and prognosis of various diseases, especially melanoma.

Keywords: Lactate dehydrogenase; Melanoma; Nicotinamide adenine dinucleotide; Silicon quantum dots.

MeSH terms

Fluorescent Dyes
Humans
L-Lactate Dehydrogenase
Quantum Dots*
Silicon
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
L-Lactate Dehydrogenase
Silicon