Isolation and identification of beneficial orchid mycorrhizal fungi in Paphiopedilum barbigerum (Orchidaceae)

Fan Tian; Xiao-Feng Liao; Lian-Hui Wang; Xin-Xiang Bai; Yan-Bin Yang; Zai-Qi Luo; Feng-Xia Yan

doi:10.1080/15592324.2021.2005882

Isolation and identification of beneficial orchid mycorrhizal fungi in Paphiopedilum barbigerum (Orchidaceae)

Plant Signal Behav. 2022 Dec 31;17(1):2005882. doi: 10.1080/15592324.2021.2005882. Epub 2021 Dec 16.

Authors

Fan Tian¹, Xiao-Feng Liao², Lian-Hui Wang¹, Xin-Xiang Bai³, Yan-Bin Yang¹, Zai-Qi Luo¹, Feng-Xia Yan¹

Affiliations

¹ Guizhou Academy of Forestry, Guizhou, China.
² Guizhou Academy of Sciences, Guizhou, China.
³ College of Forestry, Guizhou University, Guizhou, China.

Abstract

Seed germination and seedling development in nearly all orchid species rely on a symbiotic relationship with mycorrhizal fungi; however, this is not the case with all mycorrhizal fungi. This study aims to provide an understanding about the important role of mycorrhiza in seed germination and growth of Paphiopedilum barbigerum. Therefore, we isolated and identified endophytic fungi from the roots of wild P. barbigerum. The beneficial mycorrhizal fungi Epulorhiza sp. FQXY019 and Tulasnella calospora FQXY017 were screened by seed symbiotic germination tests and found to promote seed germination. However, only the seeds inoculated with FQXY019 progressed from the seed germination to rooting stage. This shows that mycorrhizal fungi and P. barbigerum have a specific relation at different growth phases. In addition, we selected FQXY019 and inoculated it into MS medium, B5 medium, OMA medium, and PDA medium. The results showed that FQXY019 co-cultured on PDA significantly promoted the increase in seedling fresh weight, leaf length, and root length (p < .01). Furthermore, it significantly promoted the root number and leaf number of seedlings compared with those co-cultured on MS, B5, and OMA media and control (p < .05). Thus, this study demonstrated the promoting effect of Epulorhiza sp. FQXY019 on seed germination and seedling development, making it an alternative method for the artificial propagation of P. barbigerum.

Keywords: Paphiopedilum barbigerum; germination; growth; mycorrhizal fungi; seed; seedling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Germination
Mycorrhizae*
Orchidaceae* / microbiology
Seedlings
Seeds
Symbiosis

Grants and funding

This work was supported by the Project of Science & Technology of Guizhou Province [grant number: Qiankehezhicheng [2017]2524]; The Scientific Search Projects of Guizhou Forestry Bureau [2019] 02; Major science and technology projects in Guizhou Province [2019]3001;The Scientific Search Projects of Guizhou Forestry Bureau[2019008] [2019008];