Characterization of the complete chloroplast genome sequence of Gynostemma microspermum (Cucurbitaceae)

Xiao Zhang; Xiaodan Chen; Hao Zhang; Yuemei Zhao; Miaomiao Ju; Guifang Zhao

doi:10.1080/23802359.2021.2007811

Characterization of the complete chloroplast genome sequence of Gynostemma microspermum (Cucurbitaceae)

Mitochondrial DNA B Resour. 2021 Dec 10;7(1):32-34. doi: 10.1080/23802359.2021.2007811. eCollection 2022.

Authors

Xiao Zhang¹, Xiaodan Chen¹, Hao Zhang², Yuemei Zhao³, Miaomiao Ju¹, Guifang Zhao¹

Affiliations

¹ Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University, Xi'an, China.
² College of Life Sciences, Sun Yat-sen University, Guangzhou, China.
³ School of Biological Sciences, Guizhou Education University, Guiyang, China.

Abstract

Gynostemma microspermum C. Y. Wu et S. K. Chen is an endemic creeping herbaceous species mainly distributed in dense forests on limestone in northwestern China. Here, the complete chloroplast genome sequence of G. microspermum was obtained by Illumina pair-end sequencing. The circular complete chloroplast genome of G. microspermum is 158,692 bp in length and contains a large single copy region (87,452 bp), a small single copy region (19,068 bp) and two short inverted repeat regions (26,086 bp). The genome sequence encodes 133 genes including 87 protein-coding genes, 37 transfer RNA genes, 8 ribosomal RNA genes and 1 pseudogene. The maximum likelihood (ML) phylogeny estimation shows that G. microspermum is sister to all other analyzed species of the genus Gynostemma with high bootstrap support.

Keywords: Gynostemma microspermum; chloroplast genome; illumina sequencing; phylogenetic relationship.

Grants and funding

This work was supported by the National Natural Science Foundation of China [Grant No. 32000256 and No. 31900273], the Project funded by China Postdoctoral Science Foundation [Grant No. 2020M673627XB], and the Natural Science Foundation of Guizhou Education University, China [Grant No. 2019BS006].