Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery

Yuya Kumagai; Yuelin Liu; Haruyasu Hamada; Weifeng Luo; Jianghui Zhu; Misa Kuroki; Yozo Nagira; Naoaki Taoka; Etsuko Katoh; Ryozo Imai

doi:10.1093/plphys/kiab570

Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery

Plant Physiol. 2022 Mar 28;188(4):1838-1842. doi: 10.1093/plphys/kiab570.

Authors

Affiliations

¹ Genome-Edited Crop Development Group, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai 3-1-3, Tsukuba, Ibaraki 305-8604, Japan.
² Biotechnology Research Laboratories, Agri-Bio Research Center, Kaneka Corporation, Iwata, Shizuoka 438-0802, Japan.
³ Structural Biology Team, Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8602, Japan.

Abstract

Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Gene Editing
Mutation / genetics
RNA, Guide, CRISPR-Cas Systems / genetics
Triticum* / genetics

Substances

RNA, Guide, CRISPR-Cas Systems