Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR

Yi Xie; Jianxiang Wu

doi:10.1007/978-1-0716-1835-6_26

Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR

Methods Mol Biol. 2022:2400:275-282. doi: 10.1007/978-1-0716-1835-6_26.

Authors

Yi Xie¹, Jianxiang Wu²

Affiliations

¹ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
² State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China. wujx@zju.edu.cn.

PMID: 34905210
DOI: 10.1007/978-1-0716-1835-6_26

Abstract

Plant virus infections cause damages to all kinds of crops, and result in enormous economic and yield losses worldwide. Numerous reports indicated that many plant viruses are vertically transmitted through seeds, and cause severe disease symptoms on seedlings that again serve as secondary transmission sources in fields. Therefore, it is necessary to develop efficient methods to detect the seeds infected by viruses. Here, we describe a RT-PCR protocol for detection of Cucumber green mottle mosaic virus (CGMMV), a tobamovirus in cucurbitaceous crop seeds. This method can be easily adapted for diagnosis of other plant viruses such as Tomato brown rugose fruit virus in seeds.

Keywords: Cucumber green mottle mosaic virus; RT-PCR; Seed transmission; Seed-borne virus; Tobamoviruses; Tomato brown rugose fruit virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Plant Diseases*
Reverse Transcriptase Polymerase Chain Reaction
Seeds
Tobamovirus* / genetics

Supplementary concepts

Cucumber green mottle mosaic virus